Mouse IgG2b antibody [RM108]
分类: 最上层  发布时间: 2018-03-20 19:26 

Mouse IgG2b antibody [RM108]
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货号: gtx60855
应用范围: ELISA, Flow cytometry/FACS, ICC/IF, Immunohistochemistry, Immunoprecipitation, Western blot
适应物种: Mouse
宿主: Rabbit

Western blot of nonreduced(-) and reduced(+) mouse IgG2b (20ng/lane), using 0.2ug/mL of clone RM108. This antibody reacted to nonreduced IgG2b (~150 kDa) much stronger than the reduced γ2b(~50 kDa).

A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

ELISA of mouse immunoglobulins shows RM108 reacted to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM108 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

温馨提示:不可用于临床治疗。
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